Tesfay Teamir

Photo Physical Properties Caffeine and Cu (Ii)-caffeine Complex: Developing Derivative Fluorescence Techniques in Analyzing Trace Amount of Non Fluorescing Substances Tesfay Teamir

In this project UV/Vis absorption spectra of caffeine in water and dichloromethane, copper sulphate, and mixture of caffeine and copper sulphate in water solution is investigated. UV/Vis absorption spectrum of Cu (II)-Caffeine complex is also investigated as a function of time during refluxing to check complex formation. The molar decadic absorption coefficient of caffeine is calculated. Fluorescence emission spectra of Cu (II)- Caffeine complex is also investigated and compared with its absorption band. Area normalization analysis method is used. Finally, a better derivative fluorescence method for determination of caffeine content of substances is recommended.