Carlos M Palmeira

Mitochondrial Regulation: Methods and Protocols - Methods in Molecular Biology Carlos M Palmeira

Jacket Description/Back: In this volume" "expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful "Methods in Molecular Biology "series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "Mitochondrial Regulation: Methods and Protocols "seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics"Table of Contents:1. Isolation of Crud Mitochondrial Fraction from CellsMariusz R. Wieckowski and Lech Wojtczak 2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein Analyses of MicroRNA and Protein ExpressionsPedro M. Borralho, Clifford J. Steer, and Cecilia M. P. Rodrigues 3. PCR Based Determination of Mitochondrial DNA Copy Number in Multiple SpeciesRooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, Mayer GD, Greenamyre JT, and Meyer JN 4. NAD+ Content and its Role in MitochondriaWei Li and Anthony A. Sauve 5. Measuring PGC-1 and its Acetylation Status in Mouse Primary MyotubesAna P. Gomes and David A. Sinclair 6. Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes Sofia M. Ribeiro, Alfredo Gimenez-Cassina, and Nika N. Danial 7. Determination Of Oxidative Phosphorylation Complexes ActivitiesJoao S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo 8. Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial DefectsAgnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski 9. A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies Fabrice Rappaport 10. BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial FunctionElena Silvestri, Assunta Lombardi, Federica Cioffi, and Fernando Goglia 11. Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus MitochondriaKieran J. Clarke, Audrey M. Carroll, Gemma O Brien & Richard K. Porter 12. Exploring Liver Mitochondrial Function By 13c-Stable Isotope BreathTests. Implications In Clinical BiochemistryIgnazio Grattagliano, Leonilde Bonfrate, Michele Lorusso, Luigi Castorani, Ornella de Bari, and Piero Portincasa 13. Following Mitochondria Dynamism: Confocal Analysis of The Organelle MorphologyF. R. Mariotti, M. Corrado, and S. Campello14. Analysis of Pro-apoptotic Protein Trafficking to and From MitochondriaIgnacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa Serafim, Vilma Sardao, and Paulo J. Oliveira 15. Mitophagy and Mitochondrial BalanceSimone Patergnani and Paolo Pinton"